Synthesis of Biomaterial-Based Hydrogels Reinforced with Cellulose Nanocrystals for Biomedical Applications

Cellulose nanocrystals (CNC) were prepared by formic acid hydrolysis and TEMPO- (2,2,6,6-tetramethyl-piperidine-1-oxyl-) mediated oxidation. The CNCs reinforced into biopolymers chitosan (CHI), alginate (ALG), gelatin (GEL) to obtain “CNC-ALG-GEL” “CNC-CHI-GEL” hydrogels. synthesized hydrogels characterized for physicochemical, thermal, structural characterization using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermal gravity analysis (TGA), X-ray diffraction (XRD) analyses. Notably, the reinforcement of CNC has not altered molecular structure a biopolymer as revealed FT-IR analysis. with have shown better stability miscibility chemical interaction electrostatic attraction between biopolymers. biocompatibility was investigated evaluating viability L929 fibroblast cell, which represents good nontoxic nature. These could be implemented in therapeutic biomedical research regenerative medicinal applications.